Alexis I. du Pont (1816-1857) was proprieter of his family business, E.I. du Pont de Nemours & Company, which began in the manufacture of gunpowder in 1802. He was the youngest child of Eleuthère Irénée du Pont (1771-1834), the founder of the DuPont Company. This collection contains thirteen photographic items, mostly portraits of Alexis I. du Pont and other du Pont family members.

Henry du Pont (1812-1889) was the second son of Eleuthère Irénée du Pont (1771-1834) and senior partner of E.I du Pont de Nemours & Company from 1850 to 1889. The collection consists of materials relating to the descendants of Henry and Louisa Gerhard du Pont. Primarily, the collection consists of portraits and other photographs of their daughters Evelina, Ellen, Louisa, Sara, Victorine, Sophie and other family members.

Karen du Pont Farquhar (1947-) is the great-granddaughter of T. Coleman du Pont (1863-1930), president of E.I. du Pont de Nemours & Company in the early 20th century. This collection of photographs is part of a larger group of du Pont family papers, which are housed in the Manuscripts and Archives Department. The photograph collection includes many photographs of the du Pont family from 1882 to 1960.

Marguerite du Pont Lee (1862-1936) was a descendant of Pierre Samuel du Pont de Nemours (1739-1817) who founded the E.I. du Pont de Nemours & Company with his son Eleuthere Irenee du Pont (1771-1834) in 1802. The E.I. du Pont de Nemours & Company is a chemical company more commonly referred to as the DuPont Company. This small collection consists of four items belonging to Marguerite du Pont Lee. Two individual portraits, a photograph of a gate, and a decorative leaf.