Alfred Irénée du Pont (1864-1935) was the eldest son of E.I. du Pont (1829-1877). He joined the family gunpowder firm in 1884, after attending the Massachusetts Institute of Technology. In the 1902 reorganization, he became co-owner and general manager. As a result of a family quarrel, he left the firm in 1916 and eventually built his own banking and real estate empire in Florida. He was an accomplished musician and played the violin, flute, clarinet, cornet, piano, and bass and founded the Tankopanicum Musical Club in 1901.

Gabrielle Shubrick Crofton (1835-1894) was the daughter of Alfred I. du Pont's first cousin, Julia Sophia du Pont (1806-1882) and Irvine Shubrick (1797-1849). She was a granddaughter of Victor du Pont (1767-1827) and great-granddaughter of Pierre Samuel du Pont de Nemours (1739-1817). She married Robert Erskine Anderson Crofton (1834-1898), an army officer, on January 7, 1864.