Samuel Francis Du Pont (1803-1865) was a Rear Admiral in the United States Navy and fought in the Mexican-American War and the Civil War. He was the fourth child and second surviving son of Victor Marie du Pont (1767-1827) and his wife, Gabrielle Joséphine de la Fite de Pelleport (1770-1837). Pierre Samuel du Pont de Nemours (1739-1817), the famous French economist and diplomat, was his paternal grandfather.

In 1815 Du Pont received a commission as midshipmen in the United States Navy. During the next thirty years he rose steadily within the ranks, becoming a Commander attached to the Pacific squadron in 1844. During the Mexican-American War he earned distinction for his defense of the California blockade.

After the Mexican War Du Pont began a decade long tour of shore duty. He was an advocate for naval modernization. In 1849 he drew up the curriculum for the Naval Academy in Annapolis. In 1855 he was appointed to the Naval Efficiency Board which was investigating nepotism and incompetence in the officer corps.

At the start of the Civil War Du Pont was appointed a senior member of the Commission of Conference to establish naval operations for the North. Du Pont was put in charge of the South Atlantic Blockading Squadron. In November 1861 he lead a successful campaign that captured Port Royal in South Carolina. Because of the success of this operation, Du Pont was promoted to Rear Admiral in July 1862. The Union remained in control of Port Royal which was the headquarters of Du Pont's blockading squadron. By late 1862 there was considerable tension building between Port Royal and Washington as pressure mounted for an attack on Charleston. The plan was to have the new ironclad monitors lead the assault. In 1863 Du Pont's fleet of ironclads were unsuccessful at taking Charleston. This defeat, in one of the most highly publicized naval battles of the Civil War, was a tremendous blow to the Union. Blame was placed on Du Pont, who was immediately relieved of his command.

Du Pont married Sophie Madeleine du Pont (1810-1888), the youngest daughter of Eleuthère Irénée and Sophie Dalmas du Pont, in 1833. They established a household at Louviers across the Brandywine River from Eleutherian Mills. They never had children. Du Pont died on June 23, 1865, while on a trip to Philadelphia.